Perfringens Agar Base, for microbiology

Application

Perfringens Agar Base has been used in the cell culture of Clostridium perfringens.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

General description

Perfringens Agar Base with the addition of selective supplement and enrichment is used for the presumptive identification and enumeration of Clostridium perfringens from food.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Preparation Note

Suspend 23.5 g of Perfringens Agar Base in 475 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Add 25 ml of Egg Yolk Emulsion (Cat. No. 17148) and additionally the rehydrated contents of 1 vial of Perfringens S.F.P. Supplement (Cat. No. 53436) or 1 vial of Perfringens T.S.C. Supplement (Cat. No. P9352). Mix well and pour into sterile petri plates.